Fig. 1

Expression of EGFR in GBM cell lines. a Western blot analysis of EGFR level in GBM cell lines. Cell lysates obtained from the U-87MG, U-251, and DBTRG-05MG GBM cell lines were subjected to Western blot analysis with the anti-EGFR antibody. The β-actin level served as a loading control. b Flow cytometry analysis of EGFR expression profile in untreated GBM cells. The indicated cells were collected and labeled the with anti-EGFR antibody and Alexa Fluore-488 secondary antibody to determine the EGFR level. Cells with EGFR signals that were more than 0.1% stronger than those of the antibody control were categorized as EGFR-positive cells, and the percentage of positive cells is expressed as the relative EGFR expression level. b’ EGFR-positive cells quantified using flow cytometry (n = 3). c IF staining of EGFR-expressing cells. The indicated GBM cells were stained with anti-EGFR (green) and DAPI (blue). Native EGFR was detected in U-251 and DBTRG-05MG cells. Green, anti-EGFR; blue, DAPI. Scale bar, 20 µm