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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Antimicrobial effects of zero-valent iron nanoparticles on gram-positive Bacillus strains and gram-negative Escherichia coli strains

Fig. 3

Analysis of RedoxSensor and propidium iodide (PI) staining intensity in B. subtilis 3610 (Bs3610) and B. thuringiensis 407 (Bt407). The two Bacillus strains were grown in MHB supplemented with ZVI NPs at concentrations of 0, 100, 500, and 1000 ppm for 3 h, and the cells were then treated with a RedoxSensor Green (green) or b PI (red). The X-axis shows the RedoxSensor or PI fluorescence intensity in arbitrary units (au), and the Y-axis indicates the cell counts as measured by flow cytometry. PBS-only and unstained cells were used as controls. Data are representative of two separate experiments. RedoxSensor fluorescence is presented in a false green color, and PI staining is presented in a false red color for clearer visualization

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