Antimicrobial effects of zero-valent iron nanoparticles on gram-positive Bacillus strains and gram-negative Escherichia coli strains

Table 1 Flow cytometry analysis of B. subtilis 3610 and B. thuringiensis 407

Strain	Concentration	PI M1 Gate % = (PI count − background)/(total count) (%)	RedoxSensor M1 Gate % = (RedoxSensor count − background)/(total count) (%)	PI Geo mean (au)	RedoxSensor Geo mean (au)
Bs3610	Unstained	0.74	1.36	22.05	328.80
	1000 ppm	14.04	78.88	40.21	1002.53
	500 ppm	1.98	95.61	65.98	488.07
	0 ppm	2.21	96.28	94.18	594.69
	PI only	0.82	2.82	32.41	429.63
	RedoxSensor only	1.09	95.06	26.47	577.59
Bt407	Unstained	1.05	3.56	23.09	580.79
	1000 ppm	4.38	96.51	93.19	1244.31
	500 ppm	3.35	97.66	90.06	783.37
	0 ppm	2.71	98.19	100.89	655.33
	PI only	2.48	4.25	54.80	412.67
	RedoxSensor only	0.23	99.60	23.03	767.53

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