Fig. 4

Flow cytometry analyses of A549 (a) and A549/ADR (b) cells following a 2-h incubation with free Rh123, P-P/Rh123, P-SS-P/Rh123, or AG-P-SS-P/Rh123. c Laser scanning confocal microscopy-based comparison of the cellular uptake of Rh123 from different formulations by A549 and A549/ADR cells (scale bar, 30 mm). d Analysis of the pathway responsible for the uptake of AG-P-SS-P/Rh123 nanomicelles by A549 cells. This analysis was performed 30 min after incubation. The cells were blocked with different inhibitors: 2 mM AG, 10 mM AG, 1 μg/mL colchicine, 0.4 μg/mL PhAsO, or 0.5 μg/mL filipin. Green, Rh123 (scale bar, 50 mm). *P < 0.05, compared with Free Rh123 in A549/PTX cells, respectively. *P < 0.05, compared with PBS, respectively. (1) Image of CLSM; (2) fluorescence intensity of image