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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Graphene oxide conjugated with polymers: a study of culture condition to determine whether a bacterial growth stimulant or an antimicrobial agent?

Fig. 3

Bacterial viability. For a, b E. coli (OD600 ~ 0.05) and B. subtilis (OD600 ~ 0.05) that are treated with POAA and chitosan and incubated with a POAA and b chitosan to achieve the concentration of 0–100 μg mL−1 with nutrient medium for 3 h at 37 °C. The incubated bacteria were then diluted to a dilution factor of 10−5–10−8 and plated on the agar plates. The plates were incubated aerobically at 37 °C for 12–16 h. After incubation, the number of surviving bacteria was measured using by colony forming unit (CFU) counting assay was determined and switched the unit to percentage (%) which corresponds to the unit of CFU mL−1 (***p < 0.0001, **p < 0.001 and *p < 0.01 obtained by Student’s t-test), cf Growth curves for bacteria. GO, GO-POAA, and GO-chitosan (GO materials, up to 20 μm wide, were delivered in a dose of 50 μg mL−1) were added to (c,e) E. coli (OD600 ~ 0.05), and d, f B. subtilis (OD600 ~ 0.05) and then incubated with either c, d nutrient or e, f PBS alone at 37 °C for 3 h. The absorbance at an optical density of 600 nm was recorded. Data are mean ± SD (n = 6)

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