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Table 6 ROS assay

From: Graphene oxide conjugated with polymers: a study of culture condition to determine whether a bacterial growth stimulant or an antimicrobial agent?

Incubate with MB medium

Incubate with PBS buffer

Concentration (μg/mL)

Loss of GSH (%) of GO

Loss of GSH (%) of GO-POAA

Loss of GSH (%) of GO-chitosan

Concentration (μg/mL)

Loss of GSH (%) of GO

Loss of GSH (%) of GO-POAA

Loss of GSH (%) of GO-chitosan

0

0 ± 2.2

0 ± 1.5

0 ± 3.1

0

0 ± 3.3

0 ± 1.8

0 ± 2.9

10

0 ± 5.1

0 ± 4.3

59.4 ± 6.2

10

0 ± 1.4

4.8 ± 2.7

80.1 ± 6.2

100

0 ± 3.2

67.3 ± 3.9

99.5 ± 2.4

100

86.5 ± 4.8

93.4 ± 5.3

99.9 ± 3.4

  1. In vitro GSH oxidation is used to determine the oxidation stress that is mediated by the GO- and GO-based materials (up to 20 μm wide) that are used to treat E. coli. Data are mean ± SD (n = 6)