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Table 7 ROS assay

From: Graphene oxide conjugated with polymers: a study of culture condition to determine whether a bacterial growth stimulant or an antimicrobial agent?

Incubate with MB medium Incubate with PBS buffer
Concentration (μg/mL) Loss of GSH (%) of GO Loss of GSH (%) of GO-POAA Loss of GSH (%) of GO-chitosan Concentration (μg/mL) Loss of GSH (%) of GO Loss of GSH (%) of GO-POAA Loss of GSH (%) of GO-chitosan
0 0 ± 3.7 0 ± 2.0 0 ± 1.5 0 0 ± 1.2 0 ± 2.6 0 ± 3.3
10 0 ± 2.6 0 ± 3.9 78.6 ± 1.8 10 0 ± 2.6 30.6 ± 3.5 85.8 ± 2.9
100 5.5 ± 1.3 79.1 ± 5.2 98.9 ± 1.2 100 95.7 ± 3.9 99.2 ± 2.8 99.8 ± 1.1
  1. In vitro GSH oxidation is used to determine the oxidation stress that is mediated by the GO- and GO-based materials (up to 20 μm wide) that are used to treat B. subtilis. Data are mean ± SD (n = 6)