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Table 7 ROS assay

From: Graphene oxide conjugated with polymers: a study of culture condition to determine whether a bacterial growth stimulant or an antimicrobial agent?

Incubate with MB medium

Incubate with PBS buffer

Concentration (μg/mL)

Loss of GSH (%) of GO

Loss of GSH (%) of GO-POAA

Loss of GSH (%) of GO-chitosan

Concentration (μg/mL)

Loss of GSH (%) of GO

Loss of GSH (%) of GO-POAA

Loss of GSH (%) of GO-chitosan

0

0 ± 3.7

0 ± 2.0

0 ± 1.5

0

0 ± 1.2

0 ± 2.6

0 ± 3.3

10

0 ± 2.6

0 ± 3.9

78.6 ± 1.8

10

0 ± 2.6

30.6 ± 3.5

85.8 ± 2.9

100

5.5 ± 1.3

79.1 ± 5.2

98.9 ± 1.2

100

95.7 ± 3.9

99.2 ± 2.8

99.8 ± 1.1

  1. In vitro GSH oxidation is used to determine the oxidation stress that is mediated by the GO- and GO-based materials (up to 20 μm wide) that are used to treat B. subtilis. Data are mean ± SD (n = 6)