Fig. 5

Kinetics of formation of the enzymatic product in serum (a) and plasma (b) for different protocols of immobilization, i.e. covalent (dashed lines) and oriented (solid lines) coupling to 100 µg/mL specific (upper lines) and unspecific (anti-PCB169 IgG, lower lines) antibodies. Rates are expressed in units of cm⁻¹ s⁻¹. In serum/plasma, the rates of enzymatic reaction are 3.8e⁻⁴/4.4e⁻⁴ cm⁻¹ s⁻¹ for oriented coupling to specific antibodies, 7.3e⁻⁵/6.9e⁻⁵ cm⁻¹ s⁻¹ for oriented coupling to unspecific antibodies, 2.2e⁻⁴/2.6e⁻⁴ cm⁻¹s⁻¹ for covalent coupling to specific antibodies and 8.1e⁻⁶/5.6e⁻⁵ cm⁻¹s⁻¹ for oriented coupling to unspecific antibodies