Fig. 5

In vivo bioluminescence images. Bioluminescence signal after completion of each treatment: saline only, PDT only, PTT only and PDT/PTT combined. The bioluminescence signal reduction suggests a possible tumoral remission during the treatments on the 4T1-Luc cells-bearing BALB/c mice (n = 5). The bioluminescence signal increase on groups PDT only and PTT only over the three treatments displayed tumor growth and metastasis, whereas in the combined PDT/PTT treatment group there was a bioluminescence signal reduction throughout the treatments. Due to the color scale, the first animal from tumor saline group, in the column 30 days from the last treatment, had its bioluminescence signal overestimated. Control group (not shown) do not presented bioluminescence signal. PDT photodynamic therapy (660 nm LED light); PTT photothermal therapy (808 nm NIR laser light)