Fig. 1

Identification of the multivalent DNA plasmid pPCFN-CpG. a Schematic representation of pPCFN-CpG. The chimeric gene containing four different virulence genes was inserted into pVAX1 utilizing HindIII and EcoRV sites. b The multivalent DNA plasmids were separated by electrophoresis. Lane 1, pVAX1 empty plasmid; Lane 2, pPCFN-CpG plasmid; Lane M, DNA marker-DL5000. c The digestion products were separated by electrophoresis. Lane 1, two fragments after restriction enzyme digestion with HindIII and EcoRV; Lane 2, pPCFN-CpG plasmid. Lane M, DNA marker-DL5000. The position of the 1482 bp band is indicated by the arrow. pPCFN-CpG, the DNA plasmid contains the epitope of plo, cbpA, fimA, and nanH gene of T. pyogenes and CpG ODN1826 motif