Fig. 3
Transient expression of the chimeric protein and activation of CpG. a HEK293T cells were transfected with plasmid pPCFN-CpG, pVAX1-PCFN, pVAX1-PCF, pVAX1-PC, pVAX1, or pPCFN-CpG-CS-NPs respectively. Transient expression of proteins was detected with anti-PLO mouse polyclonal antibody by using fluorescence microscopy assays (A3-A7). (A1) Cell controls. (A2) Cells were transfected with vector pVAX1. FITC: FITC-conjugated goat anti-mouse IgG; Hoechst: cell nuclei; Merge: the overlay fluorescence images of FITC and nucleus; Bright: the external profiles of HEK293T cells. The scale bar is 50 μm. b and c RAW264.7 were transfected with pVAX1, pVAX1-PCFN or pPCFN-CpG plasmid for 36 h. The expression levels of TLR9 and Myd88 were detected by western blotting (b) and qPCR (c). Data are shown as the mean ± SEM of three independent experiments. ***p < 0.001 and **p < 0.01