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Table 2 Primers for quantitative PCR used in this study

From: Chitosan-DNA nanoparticles enhanced the immunogenicity of multivalent DNA vaccination on mice against Trueperella pyogenes infection

Gene assayed

Primer sequence

TLR9

5′-TGGGCCCATTGTGATGAACC-3′ (forward)

5′-CTTGGTCTGCACCTCCAACA-3′ (reverse)

Myd88

5′-GCACCTGTGTCTGGTCCATTG-3′ (forward)

5′-TCTGTTGGACACCTGGAGACA-3′ (reverse)

GADPH

5′-CCCACTCTTCCACCTTCGAT-3′ (forward)

5′-CTTGCTCAGTGTCCTTGCTG-3′ (reverse)

  1. These primers were designed in this study