Fig. 5

ELP V40 diameters as measured using DLS in 1X PBS pH 7.4, upon heating (filled diamond) and cooling (square). Solution temperature was altered in 5 °C increments with a 2 min equilibration period prior to taking readings. ELP solution concentrations were a 0.05, b 0.10, c 0.50 and d 1.0 mg/mL. Missing temperature points were considered unreliable and removed. TEM was used in these instances to analyze particle diameters. Trend lines are presented only to guide the eye; each data point represents the average ± SD, n ≥ 10. *p < 0.05, **p < 0.005 and ***p < 0.0001