Table 1 Summaries of ELP assembly and disassembly behaviour upon heating and cooling as observed with DLS in 1X PBS pH 7.4 with heating and cooling in 5 °C increments
Sample (mg/mL) | Tt heating (°C) | Tt cooling (°C) | Hysteresis (∆ °C) | Zeta potential (5 °C, mV) | Zeta potential (37 °C, mV) | Notes |
|---|---|---|---|---|---|---|
L20 0.05 | 20 | N/A | 20+ | − 0.882 ± 0.8 | − 11.3 ± 0.6 | No disassembly observed |
L20 0.10 | 15 | N/A | 15+ | − 1.27 ± 0.4***a | − 11.4 ± 1.0 | No disassembly observed |
L20 0.50 | 20 | N/A | 20+ | − 7.04 ± 0.1***a | − 10.1 ± 0.4 | Not all diameters quantifiable |
L20 1.0 | 15 | N/A | 15+ | − 7.04 ± 0.5 | − 9.52 ± 0.8 | Not all diameters quantifiable |
L40 0.05 | 20 | 5 | 15 | − 3.47 ± 4.0 | − 14.0 ± 0.5 |  |
L40 0.10 | 15 | 5 | 10 | − 6.10 ± 1.9 | − 14.1 ± 1.4*b |  |
L40 0.50 | 15 | N/A | 15+ | − 0.233 ± 4.4 | − 10.4 ± 0.2*b | No disassembly observed |
L40 1.0 | 15 | N/A | 15+ | − 5.38 ± 0.2 | − 10.4 ± 0.9 | No disassembly observed |
L80 0.05 | 15 | 5 | 10 | − 9.53 ± 3.4 | − 12.2 ± 0.8 |  |
L80 0.10 | 15 | 5 | 10 | − 5.25 ± 4.4 | − 13.5 ± 2.5 |  |
L80 0.50 | 10 | 5 | 5 | − 4.22 ± 0.5*c | − 13.3 ± 1.6 |  |
L80 1.0 | 10 | 5 | 5 | − 8.89 ± 1.4*c | − 12.5 ± 1.9 |  |
L160 0.05 | 15 | 5 | 10 | − 0.242 ± 2.8**d | − 10.2 ± 1.1 |  |
L160 0.10 | 10 | 5 | 5 | − 1.71 ± 1.8 | − 10.4 ± 2.6*e |  |
L160 0.50 | 10 | 5 | 5 | − 2.37 ± 8.0 | − 15.8 ± 1.1*e |  |
L160 1.0 | 10 | 5 | 5 | − 8.06 ± 0.2**d | − 13.6 ± 0.6 |  |
V40 0.05 | 35 | 10 | 25 | 0.344 ± 3.5 | − 6.94 ± 1.6 |  |
V40 0.10 | 35 | 20 | 15 | − 0.834 ± 1.2 | − 7.00 ± 0.14 |  |
V40 0.50 | 30 | 25 | 5 | − 1.37 ± 1.1*f | − 5.73 ± 0.3 | Not all diameters quantifiable |
V40 1.0 | 30 | 25 | 5 | − 3.90 ± 0.6*f | − 5.61 ± 0.9 | Not all diameters quantifiable |