Fig. 4From: Multifunctionalized biocatalytic P22 nanoreactor for combinatory treatment of ER+ breast cancerActivation of functionalized PpIX in P22CYP-PpIX-PEG(EST) by UV365 nm (3 J/cm2) produced effective ROS. Cells were grown and incubated with the particles for 12 h, washed with PBS, treated with DCFDA for 30 min and then photo-irradiated. Green emission of DCF was analyzed by confocal microscopy and quantified by ImageJ software. Results are expressed as the mean ± SEM (n = 3). ****p < 0.0001 using Sudent’s t testBack to article page