Fig. 6

Acute toxicity and pharmacokinetic characterization of folic acid-functionalized Gd₂O₃:Eu³⁺ nanoparticles (N3-FA) in CD1 mice. a Change in body weight of CD1 mice after i.v. injection of N3-FA at different doses, b detection of N3-FA in whole blood of CD1 mice using fluorescent spectrophotometer at various time intervals, c biodistribution of N3-FA in different organs of CD1 mice 2 h after i.v. injection at a dose of 200 mg/kg, analyzed using CLSM of tissue sections, Scale bar = 50 µm, and d mean fluorescent intensity as a function of N3-FA accumulation in different organs of CD1 mice. *P < 0.05; **P < 0.01 vs kidney using 1-way ANOVA with a Bonferroni post-test