Fig. 6

Accessibility of the C-terminally located hexahistidine-peptide on chimeric HBcAg-VLPs. a Typical plot of biolayer interferometry measurement (6His construct). All measurements were performed using equal VLP-loadings of 1.6 nm ± 0.1 on APS-sensors. Green and black curves indicate the detection of intact (green) or disassembled (black) VLPs using the mAb3120 specific for assembled particles. Blue and orange curves show detection of the hexahistidine-peptide on intact (orange) or disassembled (blue) VLP with an anti-hexahistidine-peptide antibody (α-His) in a concentration dependent manner. b Bindings of the α-His antibody to chimeric VLPs purified under native conditions. Bars indicate response rates at 595 s. The response rate of 6His + 8 M urea at antibody concentration of 10 nM was set to 100% binding (dashed line) and the rate of ΔHis + 8 M urea at the same antibody concentration was set as background (backg, dotted line). Insets display mAb3120 control plots using VLPs with or without 8 M urea (same color as in a)