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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma

Fig. 2

Cellular uptake of pSiNPs and subsequent phenotypic changes in U87 GBM cells. a Cellular uptake of fluorescein labelled pSiNPs. Green: pSiNPs; red: cytoplasmic mCherry; blue: nucleus. b The viability of U87 cells exposed to pSiNPs measured by Trypan Blue exclusion assay. c MRP1 expression in U87 cells exposed to siRNA via lipofectamine (Lipo/siRNA) or nanoparticle (pSiNP) delivery by immunoblotting. d Phase contrast image of U87 cells exposed to PEI-pSiNPs carrying MRP1 siRNA and untreated at day 3 post-exposure. e The proliferation of U87 cells as measured by EdU labelling of cells in S-phase. Green: EdU positive nuclei; Blue: nuclei. f Quantitation of S-phase cell proportions. (*, #, ^, /p < 0.05 as compared to untreated, PEI-pSiNPs/ctrl siRNA, PEI-pSiNPs and Lipo/siRNA, respectively)

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