Fig. 5From: Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastomaEffect of MRP1 silencing in vivo. MRP1 mRNA and protein expression level in tumours of mice being treated with siRNA-loaded PEI-pSiNPs at 48 and 72 h post-injection (intravenous) as revealed by a qRTPCR (n = 4), b immunoblotting, and c immunohistochemistry. MRP1 mRNA expression level in d kidney (n = 2) and e duodenum (n = 2). f Immunohistochemistry of Ki67 expression in the tumour to reveal the proliferative state of U87 cells in mice receiving different treatments. g H&E staining of kidney and duodenum of mice treated with PEI-pSiNPs with MRP1 siRNA or ctrl siRNA, or none, revealing the histopathology of MRP1 downregulation in distal organs over 144 h (6 d) post-injection. (*, #p < 0.05 as compared to controls pSiNPs/saline and pSiNPs/ctrl siRNA, respectively)Back to article page