Fig. 5
a Hemocompatibility of Ln-doped QDs assayed on human RBCs. PBS (pH 7.4) and 0.1% Triton X-100 were utilized as negative and positive controls, respectively. Images on the right side show representative photographs of exposed samples. The values are expressed as the mean of three independent replicates (n = 3). b Protein corona profiles obtained after 30 min incubation of Ln-doped QDs, with human plasma followed by extensive washing, elution and loading onto 12% SDS-PAGE. MW weight marker. c Proteins were further quantified by densitometric analysis. d Representative SCGE fluorescence micrographs showing negligible genotoxicity induced by Ln-doped QDs (2 μM, 24 h) in neuroblastoma cells, negative control [PBS (pH 7.4)], positive control (150 µM H2O2). Bar graphs below depict quantitation of index of damage (comet grades). Scale bar, 50 μm. The values are expressed as the mean of three independent replicates (n = 3). Vertical bars indicate standard error. e Immunoblots of whole-cell lysates showing regulatory effects of Ln-doped QDs on expression of selected proteins