Skip to main content
Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping

Fig. 6

a Schematic depiction of a facile preparation of hNET-targeting Ln-doped QDs using cysteine-functionalized heptapeptide derived from immunoglobulin binding protein A for site-directed conjugation of anti-hNET antibodies. b Recovery of peptide linker binding onto surface of Ln-doped QDs. The values are expressed as the mean of three independent replicates (n = 3). Vertical bars indicate standard error. c SDS-PAGE showing components eluted from Ln-doped QDs. MW, weight marker. d Emission spectra of prepared constructs using λexc = 360 nm. Inserted are photographs of constructs after exposure to UV transillumination (λ = 312 nm). e TEM micrograph of anti-hNET@TbQDs. Scale bar, 100 nm. f Size distribution histogram of anti-hNET@TbQDs analysed by quasielastic DLS. Inserted is ζ-potential value analyzed in RS 100-fold diluted with Milli-Q water (pH 7.4) by Doppler microelectrophoresis. g Immunoblots of whole-cell lysates showing expression of hNET in four distinct cell lines. h Living cells fluorescence microscopy illustrating specific accumulation of anti-hNET@TbQDs (1 μM QDs upon 24 h incubation) on a surface of SH-SY5Y cells. Nuclei were counterstained with Hoechst 33342. Scale bars, 20 μm. i Immunoblots of whole-cell lysates showing stimulation of hNET in SH-SY5Y by SAHA. j Living cells fluorescence microscopy illustrating anti-hNET@TbQDs accumulation associated with SAHA-stimulated hNET expression. Nuclei were counterstained with Hoechst 33342. Scale bars, 20 μm

Back to article page