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Table 1 Apparent kinetic parameters (Km, Vmax) of HRP-antigen conjugates and Au@Pt NR-antigen conjugates

From: Highly sensitive and robust peroxidase-like activity of Au–Pt core/shell nanorod-antigen conjugates for measles virus diagnosis

Catalyst

Substrate

Km (mM)

Vmax (nM s−1)

HRP-antigen conjugates

TMB

0.15

16.12

HRP-antigen conjugates

H2O2

0.68

16.54

Au@Pt NR-antigen conjugates

TMB

0.30

195.17

Au@Pt NR-antigen conjugates

H2O2

10.67

125.65

  1. Condition: at 37 °C in 0.1 M PBS buffers (pH = 5). For TMB as the substrate, the H2O2 concentration was fixed at 20 mM. For H2O2 as the substrate, the TMB concentration was fixed at 1 mM