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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Immunoassays for scarce tumour-antigens in exosomes: detection of the human NKG2D-Ligand, MICA, in tetraspanin-containing nanovesicles from melanoma

Fig. 2

Detection of MICA-containing exosomes by ELISA, using different tetraspanin-specific antibodies. Different amounts of metastatic melanoma Ma-Mel-86c (86c) derived exosomes were analyzed by ELISA using the combinations of capture/detection antibodies indicated at the top of each plot (capture/detection). The plots represent the optical density (OD) at 405 nm. Negative controls (Blank) include capture and detection antibodies but no exosomes. Data are the mean and SEM of three independent experiments run in duplicates (*P < 0.05, **P < 0.01)

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