Fig. 5

Exosomal MICA detection in human serum by LFIA. a Optimal human serum dilution in running buffer. Different volumes of exosome-depleted human serum were run with anti-MICA detection antibody-conjugated NP, but no exosomes, to identify background signal. b Titration of exosomes for MICA detection in human serum by LFIA. Melanoma exosomes derived from Ma-Mel-55 (55) were tested without or with 25% of exosome-depleted human serum added to running buffer (55 + HS). The mixture was run in the strips using anti-CD9 as capture antibody. Scanned strips of the experiment in triplicates are shown. The position of the test (T) and control lines (C) is indicated in figure. c Quantitation of the detected signal. Signal in the test line from B was quantified using the ChemiDoc™ MP imaging system and represented as arbitrary units (a.u.). Data represent the mean and SEM of the triplicates