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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Immunoassays for scarce tumour-antigens in exosomes: detection of the human NKG2D-Ligand, MICA, in tetraspanin-containing nanovesicles from melanoma

Fig. 6

Immunocapture of proteins on exosomes requires careful optimization. Although most immunodetection techniques share a common basis, i.e. binding with a capture antibody and detection with a complementary antibody, the methodological procedure affects the ability of a reagent to bind to the sample. In ELISA, incubation with the detection antibody is done after capture of exosomes on the antibody-coated plated while, in LFIA, exosomes are mixed in solution with the antibody. Thus, in ELISA, one side of the exosome is available for binding while, in LFIA, the exosome can be completely recovered by detection antibody. As a consequence, in ELISA, more detection antibody bound to the exosome results in a higher signal while in LFIA, more detection antibody, results in steric hindrance and the impossibility of being captured

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