Fig. 5

The optical density of AuNPs-FMDV biosensor before and after PCR using UV–Vis spectrum for validation of AuNPs-FMDV biosensor PCR condition: The peaks of absorbance–wavelength of AuNPs after PCR (blue curve) have little variety from the peaks of absorbance–wavelength of AuNPs before PCR (yellow curve), both have the same peak at 520 nm of absorbance–wavelength with slight disappearance of immobilized primer peak (260 nm) due to consuming of primers in PCR reaction and AuNPs peak not affected before or after PCR