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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection

Fig. 3

a Transmission and b fluorescence intensity images of HT29-MTX cells in the presence of food-grade E171 particles close to the substratum. c Spectral image of cells/culture medium. d Spectral image of E171 particles derived from the deconvolution of the fluorescence intensity image recorded close to the substratum. eh Same as for ad for a focal plane located 24 µm from the substratum. i Normalized fluorescence emission spectra of TiO2 particles in cell culture medium (green) and HT29-MTX cells alone in cell culture medium (red) or in the presence of TiO2 particles 2 µm (black solid line) or 20 µm above the substratum (black dashed line). λexc = 364 nm. λem = 400–700 nm

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