Fig. 3

Effect of fullerenol nanoparticles on the actin cytoskeleton. a Actin filaments were clearly visible and orderly in control MDA-MB-231 cells, while fullerenol-treated cells had disrupted and reduced actin fibers. Scale bar = 20 μm. The fluorescence intensity of labeled F-actin obtained by laser scanning confocal microscopy. b Western blots of G-actin and F-actin expression in MDA-MB-231 cells. c In vitro actin polymerization assays. In control cells, the actin fibers were tight and ordered, but fullerenol treatment significantly reduced the number of actin fibers, which were also thinner and shorter. Scale bar = 10 μm