Fig. 7
Association of fNDs with Aβ fibers and plaques. a STEM image showing fibers of Aβ and fNDs together (yellow arrows show two specific fNDs, as examples). Regions without fibers show almost no fNDs. b The image shows the hippocampus of AD mouse brain tissue slides stained for detection of Aβ plaques with the anti Aβ antibody 4G8 and Alexa 488 (green) secondary antibody; and zoomed images showing fNDs illuminated with 532 nm laser. The first inset shows a confocal image zoom of 50 × 50 um2 near an Aβ plaque. The second inset shows a confocal image zoom 5 × 5 um2. Finally, a typical emission spectrum of a fND detected under 532 nm excitation near an Aβ plaque is shown