Fig. 5
From: Targeting extracellular vesicles to injured tissue using membrane cloaking and surface display
Biodistribution and heart scar localization of targeted CDC-EV. a Schematic outlining experimental approach. b Graphical representation of whole organ Qdot 655 fluorescent measurements to identify CDC-EV biodistribution in ischemia/reperfusion (I/R) rat study animals. c Xenogen whole heart images for Qdot 655 localization of control (Ctrl) and ischemic peptide-targeted (Isch) CDC-EV. d Example TTC stains of whole heart slices to identify I/R scar location in rat hearts (blanched regions, left panels) and detailed microscopic fluorescent imaging data (right panels) of slides with thin sections of the same heart tissue to image localization of control CtrlCDC-EV + Qdot 655 or ischemia-targeted exosomes (IschCDC-EV+Qdot 655). Regions of heart scarring due to infarction in TTC stains are outlined in white and corresponding areas in heart tissue sections outlined in yellow. Scar bar indicated at 2000 μm, LV left ventricle, RV right ventricle. n = 3 rats per sample group, individual rats indicated by a circle