Fig. 7
From: Targeting extracellular vesicles to injured tissue using membrane cloaking and surface display
Homing of ischemic peptide HEK-EV using surface display. a Schematic of the lentivector expression cassette to make the fusion Ischemic peptide (Isch) coding sequence (3 repeats) fused upstream to the C1C2 domain of the human lactadherin protein (for EV membrane surface display) along with a C-terminal DDK flag tag (to detect by Western blot). b Western blot data confirming the expression of the fusion surface display protein in cells and on secreted exosomes. c Pooled FACS data of HEK-EV+GFP uptake assays with NRVM oxidative stress assays. d Immunofluorescent rat heart tissue section images from I/R models of myocardial infarction. Left ventricle (LV), right ventricle (RV) are labeled and ischemic zone (IZ) areas are encircled and labeled. n = 2 rats per experimental group, all rats received I/R injury