Fig. 2

Protein fishing from HeLa cells. Sampling of HeLa cells 24 h after actinomycin D treatment. a Cytoplasmic or b nuclear extract was deposited on grid-marked, APTES-coated coverslips, followed by protein detection using a cocktail of anti-β-actin-FITC and anti-p53-APC. The coverslips were imaged under the FITC and APC filter channels, sequentially