Fig. 3From: Protein fishing from single live cellsStandard curve generation for β-actin. a A standard curve for β-actin was generated by the aspiration-and-deposition of different volumes of pure β-actin solution on a coverslip, and b correlating the number of spotted molecules (gauged from the height of solution in the nanopipette) with the fluorescence intensity of FITC-anti-β-actin, which was used to stain the spotBack to article page