Table 1 Physicochemical characteristics of blank and mRNA complexed nanoparticles (NPs) with the percentage encapsulation efficiency (%EE) of mRNA
Nanoparticles | Size (nm) | PDI | ζ-potential (mV) | %EE |
|---|---|---|---|---|
Blank LPNs | 231 ± 7.0 | 0.09 ± 0.02 | 16.25 ± 3.24 | – |
*mRNA:LPNs_1:10 | 322.2 ± 26.1 | 0.21 ± 0.02 | − 0.05 ± 6.54 | 91.3 ± 1.5 |
*mRNA:LPNs_1:20 | 217.5 ± 11.6 | 0.16 ± 0.04 | 19.62 ± 1.13 | 87.0 ± 2.0 |
*mRNA:LPNs_1:30 | 243.5 ± 45.3 | 0.18 ± 0.08 | 22.97 ± 0.68 | 92.8 ± 2.0 |
Blank CS-PLGA NPs | 141.2 ± 6.2 | 0.12 ± 0.01 | 25.20 ± 3.84 | – |
*mRNA:CS-PLGA_1:10 | 176.4 ± 5.5 | 0.32 ± 0.00 | 13.30 ± 2.76 | 87.0 ± 0.4 |
*mRNA:CS-PLGA_1:20 | 158.6 ± 1.7 | 0.23 ± 0.00 | 20.42 ± 1.22 | 95.4 ± 0.6 |
*mRNA:CS-PLGA_1:30 | 151.3 ± 3.4 | 0.18 ± 0.02 | 25.55 ± 1.22 | 94.2 ± 0.3 |