Fig. 1
From: Combustion-derived particles inhibit in vitro human lung fibroblast-mediated matrix remodeling
Impaired human lung fibroblast displacements in 3D collagen type I hydrogels induced by CDPs. Human lung fibroblasts (MRC-5 cell line) were exposed for 24 h to 20 µg/cm2 of three different types of CDPs at 37 °C and embedded in 3D collagen type I hydrogels to study their ability to generate matrix displacements. Representative 3D displacements induced by fibroblasts (cell body in white) incubated with a culture medium (NC, negative control), bounding box 172 × 172 × 21 µm3; b ufPL, bounding box 173 × 172 × 46 µm3; c ufP90, bounding box 167 × 170 × 11 µm3; and d CCB, bounding box 172 × 172 × 26 µm3; obtained from the registration of fibril-based images. Note, on the right some displacements of a lower localized cell are visible. e Data analysis of the 3D distribution of matrix displacements under the different conditions. Data are represented as box-plots (medians and quartiles; n = 4). Statistically different from control *p < 0.005; **p < 0.0005; ***p < 0.0001