Table 1 Real-time polymerase chain reaction primers used in this study
Gene | Accession number | Primers (up: forward; down: reverse) | Product size (bp) |
|---|---|---|---|
GAPDH | NM_017008.4 | GCAAGAGAGAGGCCCTCAG TGTGAGGGAGATGCTCAGTG | 74 |
OCN | NM_013414.1 | TCACTCTGCTGGCCCTGACT CCCTCCTGCTTGGACATGAA | 100 |
ALP | NM_013059.1 | CATCATCATGTTCCTGGGAG GACCTGAGCGTTGGTGTTGT | 163 |
Col-I | NM_053304.1 | GGCGAGTGCTGTCCTTTCTG CTTCCCCATCATCTCCGTTCTCTTCC | 434 |