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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: The impact of nanoparticle-driven lysosomal alkalinization on cellular functionality

Fig. 2

a Heat maps of the high content imaging data obtained for MSC (left), or Beas2B cells (right) exposed to various concentrations (10–200 µg/ml) of Au (top half) or SiO2 (bottom half) NPs for 24 h and analyzed for relative cellular health (Viab), membrane damage (MD), mitochondrial ROS (ROS), mitochondrial health (MitoStress), cell area (Area), cell skewness (Skewness), the level of autophagy, size of the endosomal network (Endo size), average endosomal pH (Endo pH) and total size of cellular focal adhesion complexes (FAC). Data are shown as relative values after z-normalization compared to untreated control cells (= 1) where the fold-change is indicated by the respective color-code. Data have been acquired for a minimum of 5000 cells/condition which were gathered from three independent experiments. b Representative high content images of MSCs either unlabeled (0 µg/ml) or labelled with Au (top row) or SiO2 (bottom row) NPs at the concentrations indicated for 24 h. Cells were stained with Live Dead dead cell stain (green) and MitoTracker Red CMXRos (red). Scale bars: 100 µm

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