Assessment of in vitro particle dosimetry models at the single cell and particle level by scanning electron microscopy

Table 1 Physico-chemical characterization of particles

SiO₂ particle	D_SEM (nm)	D_DLS (nm) (0 h, RT)		ζ-Potential (mV)
SiO₂ particle	D_SEM (nm)	CCM	H₂O	ζ-Potential (mV)
100 nm NP	90 ± 8	173 ± 5	112 ± 2	− 29 ± 1
200 nm MP	188 ± 12	251 ± 6	188 ± 3	− 22 ± 1
500 nm MP	437 ± 23	532 ± 70	491 ± 17	− 40 ± 1

Nominal diameters are 100, 200 and 500 nm, as provided by the supplier. Diameter as observed by scanning electron microscopy (D_SEM), hydrodynamic diameter (D_DLS), in cell culture medium (CCM) and water (H₂O), and ζ-potential
NP nanoparticle, MP microparticle, DLS dynamic light scattering, SEM scanning electron microscopy

ISSN: 1477-3155