Fig. 3

Generation and characterization of siGRP78 modified exosomes. a Western blot showed the exosomes from transfected or not with siGRP78. Protein levels of the two exosomes, CD63, CD81 and Alix were evaluated. b Exosome size distribution was determined by NTA. c Electron microscopy showed the morphology of BM-MSCs-derived exosomes. Scale bar = 500 nm. d Confocal microscopy showed PLC cells treated with 10 μg/ml of control exosomes derived from BM-MSCs and siGRP78 modified exosomes from BM-MSCs. Cytoskeleton were stained with phalloidin-TRITC (red); exosomes were labeled with PKH67 (green). Scale bar = 50 μm