Fig. 3

Location of PEG-HGNs in exosomes derived from MSCs. a Intracellular localization of HGNs and PEG-HGNs in MSCs by confocal microscopy. Most of the HGNs and PEG-HGNs co-localized with exosomes stained with CD63 antibody. There was also a smaller fraction of NPs inside the cytoplasm without co-localization with exosomes. Yellow represents nuclei stained by DAPI, blue are the exosomes labeled with CD63-488 antibody, and NPs were directly visualized by reflection. b TEM images of exosomes purified (ultracentrifugation) from MSCs incubated with PEG-HGNs for 48 h and size distribution histogram. Most exosomes were loaded with NPs. c Western Blot of MSCs derived exosomes with or without PEG-HGNs. d Total protein exosomes quantification by BCA assay when cells were treated under the presence or absence of PEG-HGNs. e NTA measurements of exosomes sizes and concentrations