Fig. 6

Grafting of tri-mmanose motifs on chitosan NCs modulates the response of M. tuberculosis-infected \({\text{M}}{\upphi }{\text{s}}\). a \({\text{M}}{\upphi }{\text{s}}\) were infected, or not, with M. tuberculosis and then treated with 100 µg/ml fluorescent CS-NC-tri for 18 h. Internalization of the CS-NC-tri was then assessed by FACS and the results expressed as the percentage uptake relative to uninfected cells treated with fluorescent CS-NC-tri (controls). The results are presented as the mean ± SD of three independent experiments. b Venn diagram showing differentially-expressed genes by M. tuberculosis-infected \({\text{M}}{\upphi }{\text{s}}\) treated with CS-NCs or with CS-NCs-tri, as in Fig. 5b. c MA plot showing differentially expressed genes following CS-NC-tri treatment relative to untreated infected controls. Genes with an FDR < 0.05 are shown in red. d Graph of significantly enriched biological processes (KEGG analysis) for genes up-regulated (left graph) or down-regulated (right graph) by CS-NC-tri treatment in M. tuberculosis-infected \({\text{M}}{\upphi }{\text{s}}\). The gray line indicates − log of p = 0.05. met. metabolism, phosph, phosphorylation, sign. path. signaling pathway