Fig. 6

In vitro retention of MNP-loaded cells in flow chamber assays. a Experimental set-up for determining the magnetic in vitro retention of MNP-loaded cells. A special two-magnet system was made to apply a homogeneous magnetic gradient and ibidi flow chambers were modified to adapt them to this device. b Summary of the properties of the permanent neodymium magnets used. Displacement in the magnetic force direction (Y-axes) of MNP-loaded c Jurkat and d murine primary T cells for varying MNP concentrations and magnetic force conditions. Cell displacement was analysed in at least 100 cells per movie by Imaris software. Data (mean ± SD) are representative of three independent experiments. Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. e Capture of a flow chamber assay movie before and after magnetic field application. Arrows indicate the cell retention in the chamber wall near the magnet while applied