Fig. 7
In vivo homing capacity of Jurkat and murine primary T cells after MNP treatment with and without an EMF. a Experimental set-up for determining the homing capacity of MNP-loaded cells compared to MNP-free cells. A mixture of differentially fluorescence-labelled MNP-free and MNP-loaded Jurkat or murine T cells (107 cells; ratio 1:1) was prepared and intravenously injected into nude (Jurkat) or C57BL/6J (murine T cells) recipient mice. After 1 h, peripheral (PLN) and mesenteric (MLN) LNs and spleen were collected, processed and analysed by flow cytometry. Homing capacity of MNP-free and MNP-loaded b Jurkat and c murine T cells in the absence of an EMF, 1 h after cell injection. Data (mean ± SD) are representative of three independent experiments (n = 6). Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. Ratio of MNP-free and MNP-loaded d Jurkat and e murine T cells in the LN exposed to an EMF to control LN (no EMF), 20 min after intravenous injection of the cell mixture into recipient mice, normalized to the input ratio. f Ratio of MNP-free murine T cells, administered alone as control, in the LN exposed to an EMF to control LN (no EMF) after intravenous injection. Ratios of cell homing in magnet LN to no magnet LN (mean ± SD) are representative of three independent experiments [n = 4 (Jurkat cells), n = 6 (murine T cells)]. Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001