Fig. 9
In vivo behaviour of MNP-free and MNP-loaded murine primary T cells in the popliteal LN in the absence or the presence of an EMF. a Experimental set-up of the 2PM assays, showing the exposure of the popliteal LN to the different EMF conditions. b Capture of the 2PM movies after intravenous injection of a mixture of 107 cells, differentially labelled MNP-free (blue) and MNP-loaded (red) murine T cells into C57BL/6J recipient mice, in the absence of an EMF and in the presence of one or two magnets. HEVs are labelled in grey. c Quantification of cell speed in all conditions with Imaris software. Data (mean ± SD) are representative of two or three independent experiments. Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. d Summary of the analyses of 2PM movies. Ratio (%) of unloaded to MNP-loaded cells and cell speed (mean ± SD) in the popliteal LN are representative of two or three independent experiments