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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori

Fig. 3

In vitro cellular uptake of NE-P22 and induction of bone marrow dendritic cells maturation. a In vitro confocal fluorescence imaging of BEAS-2B cells treated 1 h with P22 or NE-P22, PBS was used as control, P22 was labeled with FITC (green fluorescence) and nuclei were stained with DAPI (blue fluorescence) (Scale bar, 50 μm). b Uptake of FITC-labeled P22 by BEAS-2B cells were analyzed using flow cytometry (n = 3). c–e Relative proportions of mice bone marrow dendritic cells positive for (c) CD40, d CD80 and e CD86 surface markers were measured using flow cytometry. The data are expressed as the mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001

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