Fig. 5From: Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pyloriAntigen uptake in the nasal septa. Micrograph of frozen sectioned nasal septal tissue isolated from mice immunized with a, b NE-P22 or c, d P22 alone, P22 was labeled with FITC. Features indicated: septal columnar epithelium (between dashed lines), basal lamina (dash-dotted line), epithelial-luminal junction (dashed line), and nasal lumen asterisk. a and c are light micrographs, b and d are corresponding fluorescence micrographs (Scale bar, 50 μm). e, f BALB/c female mice were immunized intranasally with PBS, P22-FITC, or NE-P22-FITC. The single cell suspensions were prepared from nasal tissues on 6 h post immunization. The isolated cells were stained with PerCP/Cy5.5-anti-mouse CD11c mAb or respective isotype control. P22-FITC uptake was analyzed by flow cytometry. The data are expressed as the mean ± SD (n = 3). ***P < 0.001Back to article page