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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori

Fig. 5

Antigen uptake in the nasal septa. Micrograph of frozen sectioned nasal septal tissue isolated from mice immunized with a, b NE-P22 or c, d P22 alone, P22 was labeled with FITC. Features indicated: septal columnar epithelium (between dashed lines), basal lamina (dash-dotted line), epithelial-luminal junction (dashed line), and nasal lumen asterisk. a and c are light micrographs, b and d are corresponding fluorescence micrographs (Scale bar, 50 μm). e, f BALB/c female mice were immunized intranasally with PBS, P22-FITC, or NE-P22-FITC. The single cell suspensions were prepared from nasal tissues on 6 h post immunization. The isolated cells were stained with PerCP/Cy5.5-anti-mouse CD11c mAb or respective isotype control. P22-FITC uptake was analyzed by flow cytometry. The data are expressed as the mean ± SD (n = 3). ***P < 0.001

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