Fig. 6

NE-P22 stimulated stronger specific Th1 responses. Comparison of the release profile of a IL-4, b IL-17A, c IL-22 and d IFN-γ in the supernatant of the ex vivo stimulated splenocytes of mice intranasally immunized with indicated preparations. e Representative ELISPOT results of the indicated preparations. f The number of P22 specific IFN-γ-producing cells in the spleen after immunized with indicated preparations. The results obtained were compared with CpG. The data are expressed as the mean ± SD (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001