Fig. 5

A The ischemic area of rats treated with indicated formations (0.9% NaCl (Sham), SPC, OEA and SPC, OEA, OEA–SPC, and OEA–SPC NPs, [OEA] = 10.0 mg/kg). Rats were sacrificed 21 days after reperfusion and immunohistochemistry was performed to identify the phenotype of NeuN-positive cells in cortex (B) and hippocampal CA1 (D). Quantitative analysis of NeuN + cells in cortex (C) and hippocampal CA1 (E). “a–e” represent Sham, MCAO, OEA, OEA–SPC, and OEA–SPC NPs, respectively. Data are expressed as mean ± SD. n = 10–12 rats per group. Bar is 100 µm. *P < 0.01 vs. MCAO group