Table 1 List of primary antibodies (with the appropriate dilutions) applied in western blotting and list and sequence of primers used for qRT-PCR
Antibody | Manufacturer and cat no | Dilution |
|---|---|---|
Grp94 | Santa Cruz sc-13119 | 1:1500 |
Grp78 | Santa Cruz sc-376768 | 1:250 |
GADD153 | Santa Cruz sc-7351 | 1:200 |
P-glycoprotein | Santa Cruz sc-55510 | 1:500 |
EDEM | Santa Cruz sc-377394 | 1:200 |
Cytochrome c | Abcam ab13575 | 1:500 |
LC3-A/B | Cell signaling 12741 | 1:2000 |
Na+/K+ ATPase | Santa Cruz sc-21712 | 1:200 |
α-tubulin | eBioscience 14-4502-82 | 1:1000 |
Primer | Forward | Reverse |
|---|---|---|
Grp94 | CAGTTTTGGATCTTGCTGT | CAGCTGTAGATTCCTTTGC |
Grp78 | TGTTCAACCAATTATCAGCAAACTC | TTCTGCTGTATCCTCTTCACCAGT |
GADD153 | GGAGCATCAGTCCCCCACTT | TGTGGGATTGAGGGTCACATC |
EDEM | TTGACAAAGATTCCACCGTCC | TGTGAGCAGAAAGGAGGCTTC |
GAPDH | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG |