Fig. 2

Delivery of NL@Cas9-RNP particles in a cell. a Schematic diagram describing the location of sgRNA to target DPP-4 gene locus. b CLSM analysis of NL@Cas9-RNP cell uptake after labeling Cas9 with RITC to display protein distribution. Blue (DAPI) indicates the nucleus; Red (RITC-labeled Cas9-RNP) and green (anti-Cas9) are for Cas9 protein; Yellow arrow indicates co-localization of Cas9 and DAPI (i.e., overlapping of green and blue) which provides evidence for the penetration of Cas9 complex into the nucleus; White arrow indicates the absence of Cas9 protein. c Representation of sequence deletion at human DPP-4 gene locus in SNU398 cells after gene editing by Bigdye-terminator sequencing